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Total RNA was extracted using the Nucleo Spin RNA kit (740955.50, MACHEREY-NAGEL, Dren, Germany) according to the manufacturers instructions

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[14] Later development in Season 9 [edit] Three years after Spender had been written out of the series and actor Chris Owens had moved to Toronto, Ontario, Canada, Owens received an unexpected phone call from David Duchovny, who said that The X-Files ' production crew was filming the series' finale as well as another episode late in the season, and that he wanted to bring Spender back for these two episodes

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