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Enzyme activity assays Preparation of Crude Enzyme Extract: Take (2.5 0.1) g of tobacco powder, add it to 30 mL of PBS, perform shaking extraction at room temperature for 1 h, centrifuge at 7000 r/min for 15 min, separate the supernatant, and obtain the crude enzyme extract.The enzyme activity of each sample is calculated based on the enzyme activity of the crude enzyme extract per gram of absolute dry tobacco powder (DW) (U/g)