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For example, Pseudomonas produce AHL-type QS molecules that regulate the secretion of cellulases and proteases, which are critical for macromolecular degradation and aroma precursor formation ( Staphylococcus use AIP-mediated quorum sensing to modulate biofilm formation and metabolic product synthesis, affecting aroma compound accumulation and microbial colonization ( Aspergillus secrete fatty acid-derived QS signals that coordinate the expression of lipase genes, promoting lipid degradation and volatile ester formation ( 5.4 Analysis methods of microbial interactions Currently, the main method for analyzing potential microbial interactions in cigar tobacco leaf fermentation primarily relies on Spearman correlation and co-occurrence network analysis of amplicon or metagenomic sequencing data ( Staphylococcus and Corynebacterium generally showed negative correlations with other bacteria, while Curtobacterium mainly had positive correlations with other bacteria

Depressive symptoms were assessed using the Korean version of the self-administered 21-item Beck Depression Inventory (BDI) 30

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