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Interestingly, immunoblot analysis indicated an increase of PINK1 expression levels in the mitochondria at 6 h after HS, and an increase in the translocation of Parkin from the cytoplasm to the mitochondria in vivo and in vitro ( Figures S1C, D ), suggesting that HS stabilized PINK1 at the outer mitochondrial membrane and thereby recruits more Parkin to mitochondria