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A Wisconsin and Illinois cohort series proposed diagnostic criteria for EVALI, which include use of an e-cigarette or related product in the previous 90 days, lung opacities on chest X-rays or CT scan, exclusion of infection, and absence of other likely alternatives such as heart failure or connective tissue disease.[5] In a recent Centers for Disease Control and Prevention report, more than 2800 cases of EVALI and more than 60 related deaths were described.[5] The most common symptoms were cough, shortness of breath, and fatigue

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To obtain airway epithelium by brushing, disposable brushes (2.0 mm, Ballard Medical, Draper, UT) were advanced through the working channel of the bronchoscope and used to brush and collect airway epithelial cells from 2 nd or 3 rd order bronchi from the right lower lobe by gently gliding the brush back and forth 10 times as previously described [3], [5], [41]