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Materials and methods Strains and culture conditions Yeasts were maintained and grown either in YPD broth [1% yeast extract (Oxoid), 2% bacteriological peptone (Oxoid), 2% D-glucose], or in YNB broth [0.69% yeast nitrogen base without amino acids (Formedium), 2% D-glucose] supplemented as required with amino acids, uracil, 5-FOA (Melford), D-histidine (Sigma) or reduced GSH (Sigma)

Czysto kadego peptydu weryfikowana metod HPLC 98%, tosamo obu czsteczek potwierdzona spektrometri masow Q-TOF, COA dostpne dla kadej partii (z osobnym oznaczeniem zawartoci peptydu i miedzi w kompleksie GHK-Cu)

Synapse loss is widespread within the AD-dementia, but not AD-resilient, brain