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Cytokine assay Levels of IL-1, IL-6, IL-22, IL-23, IFN-, CXCL1, CXCL2, CXCL5, CXCL17, TNF- and Resistin were determined in BAL, lung and serum by enzyme-linked immunosorbent assay (ELISA) using the manufacturers recommendation (R&D systems, Biotechne, Minneapolis, MN, United States)