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Flow cytometry For CD3/CD4/CD8a antibody staining, 2 10 5 cells were preincubated with 2.5 l Human TruStain FcX Fc Receptor Blocking Solution (#422302, BioLegend, San Diego, CA, USA), followed by incubation with the designated antibody (Brilliant Violet 421 antihuman CD3 Antibody (#317344), PerCP antihuman CD4 (#300528), Brilliant Violet 421 antihuman CD8a (#301036), and the respective isotype controls (BioLegend, San Diego, CA, USA) for 30 min on ice while being protected from light

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