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Indeed, the trend we noted at the end of the ad lib use period did show that nicotine concentrations appeared to be continuing to rise and smoking urge appeared to be continuing to decrease

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However, given that values still mostly remain in the normal reference range, future studies should investigate the effect of cigarette smoking on sperm function at the molecular level. This leads to the question, does smoking impact sperm quality in ways that may not be recognized using the semen analysis

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