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Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed with the PowerUp SYBR Green Master (Applied Biosystems) on a 7500 Fast thermocycler (Applied Biosystems)

To ensure the stability and effectiveness of blue copper peptides in skin care products, there are several key factors to consider: pH value control: GHK-Cu is unstable in a strongly acidic environment, so it needs to maintain a suitable pH value, usually a neutral or near-neutral environment, to maintain its structural integrity and biological activity

However, due to the poor methodological quality of these studies, no meaningful conclusions about the overall efficacy of B12 could be reached

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