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The fixative was removed and 150 l of Nile Red solution (6 l of Nile Red 0.5 mg/ml in acetone per 1 ml of 40% isopropanol) was added to the worms for 30 min at 20 C with gentle rocking in the dark

Others argue that improvements in posture, workout routines, and normal body development could explain any differences

However, the binding affinities of EGCG to HIF-1 and many other targets are higher than the EGCG plasma peak level in experimental animals administered with high dose of EGCG, raising a concern whether the microRNA regulation by HIF-1 is involved in the anti-cancer activity of EGCG in vivo