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Membranes were incubated with primary antibodies overnight at 4 C, followed by incubation with secondary antibodies conjugated with either horseradish peroxidase or fluorescent dye

Even so, the mechanistic arguments above still apply: IV glutathione does not engage the PCSK9 pathway, CYP metabolism, or IgG catabolism

HEK293T cells ( Homo sapiens , female, embryonic kidney cells, ATCC, CRL-1573) were cultured in high-glucose DMEM (Lonza, BE12-604F), 10% FCS (Biochrome, S0115), HEPES (10 mM, Biochrome, L1613), non-essential amino acids (1 mM, Gibco, 11140-035), 100U/ml penicillin and 100 g/ml streptomycin (1% Biochrome, 0257 F) and 2 mM sodium pyruvate (1% Biochrome, L 0473)

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