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Gene knockout by CRISPR/Cas9-sgRNAs The sgRNA expression plasmids targeting human SLC13A3 (sgRNA: GATGGTGACAATAGCCACGC) or mouse Slc13a3 ( sgRNA: GGAAGGAGGATGAATATCGT) were constructed by ligating the corresponding annealed guide oligonucleotides to pLenti-U6-sgRNA-EFFS-spCas9-P2A-Puro plasmid co-expressing the Cas9

doi: 10.1016/j.semcancer.2013.05.006 136 ChenEIHewelJKruegerJSTirabyCWeberMRKralliAet al

Even in genetically identical cancer cell populations, this process leads to the spontaneous emergence of subpopulations with distinct epigenetic profiles, forming potential seeds for resistance [199,200,201]

Chemical-nutritional characteristics and aromatic profile of milk and related dairy products obtained from goats fed with extruded linseed