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Preparation of genomic DNA for next-generation sequencing and analysis of pooled CRISPR screening Genomic DNA of LDLR high cells and unsorted control cells were extracted respectively using cell lysis buffer (400 mM Sodium chloride, 10 mM Tris, 2 mM EDTA, 0.5% SDS, pH 8) and then subjected to the established procedures 100

Reversible grade 4 hyperbilirubinemia in a patient with UGT1A1 7/7 genotype treated with irinotecan and cetuximab

7.5 Regulatory and biosafety considerations Regulatory oversight of genetically modified (GM) cultivars focuses on evaluating their safety and ensuring compliance with international standards, including those established by the Codex Alimentarius (Goodman, 2014)