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Description

Tong HC, Gao XJ, Dong XZ

doi: 10.1080/15548627.2022.2109286 14 DavisC

Sample preparation of proteins in fractionated organelles for quantitative LCMS/MS analysis The proteins in the resulted lysate were collected by acetone precipitation and dissolved in 25.5 l of 50 mM ammonium bicarbonate buffer, and then 1.5 l of 100 mM DTT in 50 mM ammonium bicarbonate buffer was added to the solution before incubation at 60 C for 30 min