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If your downstream purpose doesnt require native, functional antibodies for example, you were just quantifying antibody levels from different conditions then denaturing elution would work just fine for that purpose

Qualitative and quantitative assessment of meningococcal antigens to evaluate the potential strain coverage of protein-based vaccines

They compared the patients (good responders versus mild responders) who had already been taking vitamin B12 shots for at least six months to twenty years [17]