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Conjugation of the thiol group of glutathione to the CDNB substrate was monitored by recording changes in absorbance at 340 nm between 0.5 and 6 min after initiating the reaction at 22C

This suggests that the effects of MG extend beyond binding to GSH and involve the inhibition of GSH resynthesis and enzymes that play key roles in its synthesis, thereby promoting ferroptosis

Timing & therapeutic window Ferroptotic cell death in acute cardiac injury occurs within a narrow time frame, making the timing of intervention critical

doi: Emmez H, Yildirim Z, Kale A, Tnge M, Durda E, Brcek A, Uanku LN, Doulu F, Kili N, Baykaner MK (2010) Anti-apoptotic and neuroprotective effects of alpha-lipoic acid on spinal cord ischemiareperfusion injury in rabbits