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Traditional detection employs 2,4-dinitrophenylhydrazine (DNPH) derivatization, forming stable adducts quantifiable spectrophotometrically or via anti-DNPH antibody Western blotting (Madian and Regnier, 2010)

Although recently there has been a development of the other methods that are more efficient according to the reports, passing 70% of the oxidization levels, via utilization of increased amount of potassium permanganate, and instead of adding sodium nitrate, it involves the addition of the phosphoric acid combined with the sulphuric acid

Carnitine deficiency and insufficiency manifest as various symptoms such as hypoglycemia, hyperammonemia, fatigue, muscle weakness and cramps, cardiac hypertrophy and dysfunction, arrhythmia, etc

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