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This section categorizes the ALP-linked fluorescence immunoassays according to the signal output methods (Scheme 1): the direct generation of fluorescent molecules or quenchers, ALP catalysis coupling with chemical reaction or enzymatic cascade reaction, enzymatic-product-regulated fluorescence of nanomaterials, enzymatic-product-induced in situ generation of fluorescent nanomaterials, and enzymatic-product-triggered aggregation-induced emission (AIE) phenomenon (Table 1)

Maybe its uneven tone, lingering dark spots, or a general lack of glow