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This low immunogenicity is attributed to their minimal expression of major histocompatibility complex (MHC-I and MHC-II), Fas ligand, and T-cell co-stimulatory molecules such as B7-1, B7-2, CD40, and CD40L [117]

The blood was centrifuged at 3,000 g for 10 min at 4 C and the serum was collected and stored at 80 C

Although many pathological stimuli can trigger necroptosis, the most typical pathway for inducing necroptosis is TNF and FAS signaling, in which CASP8 inhibition activates necrosomes (a multi-protein complex containing RIPK1, RIPK3, and MLKL) to trigger necroptosis [113]