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Increased MTHFD2 levels are critical for survival from glutamine starvation in GBM cells Next, to identify how glutamine starvation affects one-carbon metabolism, we cultured U87 and T98 cells in normal medium deprived or not of glutamine to measure the gene expression of key enzymes in the one-carbon metabolic pathway (Fig

Regarding pyruvate, recent studies have shown that the activator protein 1 (AP-1) transcription factor component c-Fos regulates chondrocyte proliferation and differentiation by balancing pyruvate flux between aerobic and anaerobic glycolysis (51)

The addition of acetone (process e) in refolding led to an instantaneous precipitation of the solubilized protein and loss of the fluorescence signal (Table 4)