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Re-expressing cell lines of HMOX2 WT, HW/GW mutant, or K68R mutant were analyzed by immunofluorescence with anti-HA, anti-Calnexin, and anti-Tom20 antibodies to visualize the localization of HMOX2, ER, and mitochondria, respectively

Numerous studies have shown that lactoferrin (Lf) receptors (LfRs), expressed on the capillary endothelial cells of the brain, transport Lf across the BBB through transcytosis [23, 24]