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Importantly, we recommend that animal and cell-based models rely on multiple risk factors to mimic the events at different stages of dry AMD, and that multiple models should be utilized to deepen the rigor beyond what a single model will provide

For the preparation of media samples for metabolomics, 10 l of media was collected and added 990 l of medium extraction buffer (80% methanol, containing 2 M d27 myristic acid)