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HE and IHC staining were performed according to the manufacturers instructions

Skin and subcutis

In three consecutive enzymatic reactions, G6PDH (reaction 1), followed by 6-phosphogluconolactonase (6PGL, reaction 2) and 6PGDH (reaction 3), glucose-6-phosphate (G6P) is catabolised supplying cells with ribulose-5-phosphate maintaining the antioxidative power by generating 2 NADPH molecules

It is important that exogenous GSH not only significantly increased the content of total glutathione (by 40%) but also increased the endogenous content of its reduced form in cardiac tissue, thus contributing to maintenance of the redox potential of mitochondria and cardiac cells in general