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After TCA precipitation, the pellet was washed with ice-cold acetone (200 L) twice, dried, and dissolved in 49.5 L of DNase buffer (40 mM TrisHCl pH 7.5, 8 mM MgCl 2 , and 5 mM DTT)

Mutagenesis In the published literature, acetaminophen has been reported to be clastogenic when administered at 1500 mg/kg/day to the rat model (3.6 times the MHDD, based on a body surface area comparison)