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Zaki AM, Nada AS, Elshahed AR, Abdelgawad NH, Jafferany M, Elsaie ML

In addition, Daubry and colleagues demonstrated anxiogenic and inflammatory effects with 200 mg.kg -1 of caffeine in sleep-deprivation rats [37]

After 24 h of incubation, the supernatant was collected to quantify lactate dehydrogenase (LDH) release using the CyQuant LDH assay kit (Invitrogen, Cat #C20300), following the manufacturers protocol