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The key transcription factors that regulate gene expression are NF-E2-related factor 2 (Nrf2) via the antioxidant response element (ARE), AP-1, and nuclear factor kappa B (NF-B)

The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates

Administered by licensed medical professionals in a sterile, spa-like setting, each session lasts 3045 minutes

Enrolled 60 acne patients on isotretinoin and randomly assigned them to receive either 3 g/day of omega-3 (EPA+DHA) or placebo for 12 weeks