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Such findings could lead to a more comprehensive understanding of how zinc affects animal health at the molecular level and provide a basis for the development of more targeted zinc supplementation strategies [173]

For the quantification of sulfur metabolites in serum, the metabolites in 20 uL of serum were extracted and derivatized by NEM in 80 L of ice-cold extraction solvent (80% MeOH: 20% H2O containing 25 mM NEM and 10 mM ammonium formate, pH = 7.0) which includes stable isotope labeled internal standards (1 M [ 13 C 3 , 15 N]-cysteine-NEM, 0.1 M [ 13 C 2 , 15 N]-GSH-NEM, 2 M [D 4 ]-Cystine, 4.6 M [ 13 C 5 , 15 N 2 ]-GSSG, 5 M [D 4 ]-Hypotaurine, and 40 M [ 13 C 2 ]-Taurine followed by incubation at 4 C for 30 min

Epidemiology of gastric cancer: Global trends, risk factors and prevention

T1-weighted SPGR scans were collected (TR/TE/TI = 8.2/3.2/900 ms, flip angle = 9, 1 1 1 mm) and used to define grey matter volumes