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Synergistic Benefits of Glutathione + Vitamin C: When combined, glutathione and vitamin C exhibit synergistic effects that amplify their individual benefits

The desalted sample was evaporated to dryness in a speedvac and then resuspended in 10 L Buffer A and analyzed by mass-spectrometry using the following LC-MS gradient: 5% buffer B in buffer A from 0-15 min, 515% buffer B from 1517.5 min, 1535% buffer B from 17.592.5 min, 3595% buffer B from 92.595 min, 95% buffer B from 95105 min, 955% buffer B from 105107 min, and 5% buffer B from 107125 min

Cytotoxin-associated gene A (CagA), a key virulence factor, undergoes phosphorylation by host c-Src/c-Abl kinases, activating oncogenic signaling pathways that induce gastric epithelial inflammation, precancerous lesions, and adenocarcinoma (Zhao et al., 2024)

The amplification program was carried out on a CFX96 instrument, and primers were designed on the based on NCBI data ( Table 1 )