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Cells (2000 cells/well) were seeded in a 96-well plate and treated with varying Metformin concentrations (040 mM) and specific Simvastatin concentrations (0-50 mol) for 48 h

Methylation: an Ineluctable Biochemical and Physiological Process Essential to the Transmission of Life

To quantify protein levels in CD8 + T cells, single-cell suspensions were stained using the LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (Thermo Fisher Scientific, 10119), followed by surface and intracellular staining with fluorochrome-labeled antibodies (Supplementary Table 1)