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Vomiting is common in the early withdrawal period

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Further, yellow-coloration of glutathione (GSH) in the pancreatic tissue as a marker of antioxidant capacity, was carried out according to the method of Ellman 27 using DTNB (2, 2-dinitro-5,5-dithiodibenzoic acid) that reacts with SH groups to produce a yellow-colored complex which is measured colorimetrically at 412 nm