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Then, 200 l of chloroform was added, the tubes were gently shaken for 15 s (without vortexing), and incubated on ice or at 4 C for 5 min

Furthermore, iron-mediated epithelial cell death promotes the secretion of various pro-fibrotic factors such as TGF-, CTGF, etc., inducing proliferation and differentiation of interstitial fibroblasts, ultimately leading to renal fibrosis