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Membranes were incubated with primary antibodies at 4 C overnight, followed by incubation with alkaline phosphatase (AP)-conjugated secondary antibodies (CST, USA) for 4 h at room temperature

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Gillissen A, Jaworska M, Orth M, Coffiner M, Maes P, App EM, Cantin AM, Schultze-Werninghaus G (1997) Nacystelyn, a novel lysine salt of N-acetylcysteine, to augment cellular antioxidant defence in vitro

There are other nutrients that are not directly involved in the chemical pathways of metabolism but are important for energy production and maintaining mitochondrial function such as