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Description

C.HughesC

Pollution and skin: from epidemiological and mechanistic studies to clinical implications

Interestingly, the methylation analysis of tissue and seminal plasma samples showed a significant difference between PRAD and BPH groups

The cDNA libraries were prepared using the High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, USA), and qRT-PCR was performed using Power SYBR Green PCR Master Mix (Life Technologies, USA) on a Quant Studio 5 real-time PCR machine (Life Technologies, USA) to determine relative fold changes in target gene expression