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This triggers downstream signaling pathways (e.g., NF-B and MAPK), driving the massive release of pro-inflammatory cytokines, including IL-1, IL-6, and TNF- (167170)

Validation at low concentration levels for analytes naturally present at high concentrations, such as Gln, Glu, and GSH, can be challenging, as the response from low-concentration SA may not differ significantly from the endogenous concentration, leading to inaccurate results