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(Gast et al., 1995)

Membranes were stained with Red Ponceau dye (Sigma) to assess transfer efficiency and total protein loading

The bilirubin is then oxidized and reduced, eventually forming stercobilin (excreted in the feces) or urobilin (reabsorbed and excreted in the urine).[ref] The deconjugation reaction is facilitated by -glucuronidase , an enzyme produced by gut bacteria