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Mice were maintained on a regular diet in a pathogen-free facility on a 12 h light/dark cycle with unlimited access to food and water

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10.3390/nu5041218 70 KolczynskaK.Loza-ValdesA.HawroI.SumaraG

Images were analyzed using the Adiposoft plug-in software in ImageJ (NIH).[22] Briefly, images were converted to 8-bit images and scald to 0.366 microns per pixel (corresponding to 20 magnification on the Leica microscope)