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Lencer (Boston Childrens Hospital, Harvard Medical School and Harvard Digestive Diseases center, United States) were cultured in MCDB 131 medium (Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Gibco), 2 mM l-glutamine (Gibco), 25 g/mL streptomycin (Gibco), 25 U/mL penicillin (Gibco), 10 ng/mL recombinant mouse epidermal growth factor (Gibco), and 1 g/mL hydrocortisone (Sigma-Aldrich), at 37 C in a humidified atmosphere of 5% CO 2

The active-site cavity, which is located between the two domains, comprises a glutathione binding site (G-site), formed mainly by the N-terminal domain, and a hydrophobic substrate-binding site (H-site), which is formed by the C-terminal domain

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The antimycobacterial effects of PZA and INH has been well established., but our lab wanted to specifically test the effects of these first-line antibiotics at 1/10 the minimum inhibitory concentration against M