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We used a degradation-focused sgRNA library 59 composed of guides targeting ~2,000 genes involved in the ubiquitin, autophagy and lysosomal (UBAL) degradation pathways and isolated cells using FACS based on the GFP:BFP ratio (Fig

10.3389/fphar.2019.01600 144 SheuS.-S.NauduriD.AndersM