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Transient stress or increased cytoplasmic TDP-43 triggers phase separation into toxic cytoplasmic droplets that block nuclear import, deplete nuclear TDP-43, and cause cell death, offering a present mechanistic model for ALS/FTD pathology (Gasset-Rosa et al., 2019)

Photo-oxidation is exacerbated during retinal degeneration and progressive loss of photoreceptors, as the choroid cannot alter the inflow of oxygen supply in response to the changing retinal environment, so the remaining photoreceptors become exposed to a higher oxygen level 22

Plasma amino acids Plasma amino acid concentrations were measured by ultra-high performance liquid chromatography (ACQUITY H-Class System, Waters Corporation, MA, USA) using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (Waters AccQ Tag assay kit, MA, USA) and norvaline (Sigma Aldrich, MO, USA) as internal standard as previously described 11

Molecular dynamics simulations demonstrated that the ALOX15-luteolin complex is stable and flexible