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The complex crystallized under sitting drop vapour diffusion against 24% PEG 3350 and 100 mM MES (pH 6.5) with a drop configuration of 2 l of complex, 1.8 l well and 0.2 l 40% tert-butanol

Proc Natl Acad Sci U S A 103: 36043609

In this study, we set out to investigate those non-engineered interactions that might guide us towards a better understanding of synthetic co-cultures in general and how to design them