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The large quantities of standard ascorbic acid that remain unabsorbed create an osmotic load in the intestine, leading to gastrointestinal discomfort [3]

On the next day, media was changed to neuronal media [DMEM-F12/Glutamax supplemented with N2, B27, 20 ng/ml BDNF (Peprotech 450-02), 20 ng/ml GDNF (Peprotech 450-10), 200 nM ascorbic acid (Sigma A4544), 1 mM dibutyryl-cAMP, and 1 g/ml Lam] and half media change was done every other day