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Observations of abnormalities in gait dynamics were not detected in Thy1 -syn mice, with studies showing modest effects of the different gait parameters in this transgenic mouse model of PD (Fleming et al., 2013

Procedure for sulfhydryl modification required reaction of rat anti-mouse CD5 or isotype IgG antibodies (Biolegend, San Diego, CA, USA) with 20-fold excessive SATA (N-succinimidyl S-acetylthioacetate) (Millipore Sigma) at room temperature for 30 min

After filtration with 0.45 m filters to remove cell debris, the medium was concentrated at 4,000 g for 1 hour by using 3 KD Ultra Centrifugal Filters (Millipore) to a desired volume

R., & Neumann,