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After 4-day culture, organoids were dissociated in organoid dissociation solution (bioGenous, E238001) for 2 min at 37 C according to the manufacturers protocol, centrifuged at 68 g for 2 min, resuspended in organoid culture medium, and cultured as described above for organoids passaging

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3729(b)(1)(A) (87 FR 79452)