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Hygiene and safety practices : The clinic should have high standards in terms of the safety and cleanliness of its facilities

This phenomenon, known as reperfusion injury, involves the oxidation of cellular components, mitochondrial dysfunction, and the activation of inflammatory pathways [91, 92]

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qPCR was performed using AceQ Universal SYBR qPCR Master Mix (Vazyme, China) as following reaction conditions: one cycle at 95C for 10 min, 40 cycles of amplification at 95C for 10 s, 58C for 15 s, and finally 72C for 20 s in a QuantStudio 3 (Thermo, USA) system