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Grx activity was determined by monitoring the consumption of NADPH at 340 nm during the GSH-dependent reduction of 8 mM -hydroxyethyl disulfide (HED) using 1 mM GSH coupled with of 0.6 units of yeast GR (Sigma) [20]

Receptor binding triggers a Gs-cAMP-PKA signaling cascade leading to GH synthesis and pulsatile release

2.6.6 Molecular dynamics simulations Based on the docking results, the small molecule-protein complex was used as the initial structure for full-atom molecular dynamics simulations using AMBER 22

[18] Moreover, G6PD is one of a number of glycolytic enzymes activated by the transcription factor hypoxia-inducible factor 1 (HIF1)