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qRT-PCR was performed in QuantStudio 3 realtime PCR system (Applied Biosystems, USA) using ChamQ SYBR qPCR Master Mix (Low ROX Premixed) (Q331-02, Vazyme)

A prior cross-sectional investigation revealed lower t-tau and p-tau levels in CSF (Mollenhauer et al., 2017)

doi: 10.3389/fonc.2023.1202117 Received 07 April 2023 Accepted 07 September 2023 Published 02 October 2023 Volume 13 - 2023 Edited by Lee Fah Yap, University of Malaya, Malaysia Reviewed by Christopher Dawson, University of Warwick, United Kingdom

Introduction Metals are an integral part of many structural and functional components in the body, and the critical role of metals in physiological and pathological processes has always been of interest to researchers