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The day of the experiment, cells were treated with test compounds for the indicated times, then harvested by trypsinization, resuspended in 500 L Hanks Balanced Salt Solution (HBSS, Gibco, Invitrogen Corp., Carlsbad, Calif.) containing either H 2 DCFDA (25 M), C11-BODIPY(581/591) (2 M) or MitoSOX (5 M) (all from Molecular Probes, Invitrogen) and incubated for 10 minutes at 37 C

The particle size, PDI and Zeta potential of DBP-Lip was measured at predetermined time points of 1, 2, 7, 14, and 28 day

Advances in RNA-based cancer therapeutics: pre-clinical and clinical implications