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For each sample, 25 l of liposomes were mixed with 25 l of the 2 x reaction buffer consisting of 10LM [ 3 H]GSSG (Figure 3a and Extended Data Figure 4l) or 40 M [ 3 H]GSSG (Extended Data Figure 7F), 150 mM KCl, and 50 mM HEPES (pH 7.4)

Our study revealed negative regulation of LRP1-binding proteins (e.g., TIMP2, FN1, SERPINE2, MMP2, DCN, C1S, C1R, and CTSD) in chondrocytes exposed to H 2 O 2 , corroborating findings from LRP1 knockdown models [85], except for PSAP, which was downregulated in our study but upregulated in LRP1 knockdown models