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Briefly, the reaction mixtures (100 l) in 100 mM Tris-HCl buffer (pH 8.0) contained 1.0 M Cp1B, 10 mM ATP, 12 mM MgCl 2 , 300 M NADH, 500 M PEP, 41 U per ml PK (Sigma), 59 U per ml LDH (Sigma) and 10 mM KCl with various concentrations (0.04 mM to 1 mM) of (2 S ,3 S )- t -ES and 5 mM l-Phe